Identification of Reducing Substances in Nephritic Urine

Analytical Methods-The total and non-fermentable reducing substances in the urine were determined by the gasometric methods of Van Slyke and Hawkins (2) and recorded in terms of glucose. Diurnal Excretion of Reducing Xubstances-In the two cases studied the daily amount of fermentable and non-fermentable reducing substances excreted was determined on several successive days. The diurnal excretion was determined on 3 hour specimens during a 24 hour period. The results are recorded in Figs. 1 and 2. The concentration of total reducing substances in the urine of Case I varied mostly between 0.84 and 0.96 per cent, with 0.12 to 0.15 per cent non-ferment,able. In Case II the total reducing substances were about 0.20 per cent, of which 0.06 per cent was nonfermentable. Decolorization and Removal of Protein from Urine-The urine samples used were slightly acid to litmus. Alcohol was added to make a 20 per cent alcoholic solution, since in such a solution the amount of reducing substances adsorbed on charcoal during decolorization is reduced from 20 per cent of the total to 3 per cent